Karachitos, A; Grobys, D; Antoniewicz, M; Jedut, S; Jordan, J; Kmita, H

[Karachitos, Andonis; Grobys, Daria; Antoniewicz, Monika; Jedut, Sylwia; Kmita, Hanna] Adam Mickiewicz Univ, Fac Biol, Inst Mol Biol & Biotechnol, Lab Bioenerget, Poznan, Poland; [Jordan, Joaquin] Univ Castilla La Mancha, Sch Med, Dept Med Sci, Albacete, Spain

It has been previously demonstrated that cytoprotective activity displayed by minocycline in the case of the yeast Saccharomyces cerevisiae cells pretreated with H2O2 requires the presence of functional VDAC (YVDAC1). Thus, we decided to transform YVDAC1-depleted yeast cells (Delta por1 cells) with plasmids expressing human VDAC isoforms (HVDAC1, HVDAC2, HVDAC3) to estimate their involvement in the minocycline cytoprotective effect. We observed that only expression of HVDAC3 in Delta por1 cells provided minocycline-mediated cytoprotection against H2O2 although all human isoforms are functional in Delta por1 cells. The observation appears to be important for ongoing discussion concerning VDAC isoform roles in mitochondria and cell functioning. (C) 2016 Elsevier B.V. and Mitochondria Research Society. All rights reserved.