Resumen |
A method for the spectrofluorimetric determination of methiciliin is proposed (lambda(ex) = 279 nm, lambda(em) = 379 nm), for concentrations between 0.030 and 10.0 mug ml-1. The method was performed in water, at pH 6.20 provided by addition of phosphate buffer solution. The values obtained for detection and determination limits were 0.0188 and 0.063 mug ml-1, respectively. The method was successfully applied to assay a commercial injection containing methicillin sodium monohydrate.
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